1327 Opsins are associated with UVR induced nevus activation

Objective:To investigate whether Opsins are associated with UVR induced nevus activation. Method: Human specimens Dispase digestion at 4°C overnight.The epidermis was discard and digested dermis 0.2% type collagenase 37°C for 2-3h to obtained cells.Culture cells medium 254 human melanocyte growth factor.Nevus divided into BRAF wild BRAFV600E mutant by Sanger sequencing.Analysis of SA-β -galactosidase activity separately.The were identified immunofluorescence staining melanA, Ki67 p16.Then,used 0.5J/cm2 irradiated per day 4 times in separately . EDU used detect the proliferation control group Irradiation group.SA-β-galactosidase senescence irradiation group. Western-blot detected opsins p16 expression Result:The dendritic, SA-β-galactosidase is blue. Can be observed that melanA expressed on cell membrane, low high p16.The rate higher than group.In group，the Con 3.9% vs 16.7%(p=0.0003).In group, 4.6% 8.4%(p=0.0028).SA-β-galactosidase showed stronger decreased compared after four days.Opsin 2 opsin 3 significantly increased Conclusion:The opsin2 opsin3 while activation ultraviolet radiation.Opsins may involved